Leading Lightâ Wnt Reporter Cell Line

Hct 116 wnt reporter stable cell line control this wnt reporter cell line is a control cell line designed to show background luciferase activity for 116 wnt tcf reporter cell line active.

Leading lightâ wnt reporter cell line.

The system contains an engineered 3t3 mouse fibroblast cell line which expresses the firefly luciferase reporter gene under the control of wnt responsive promoters tcf lef. The leading light wnt reporter cell line is the basis for a cell based luciferase activity test suitable for a multi well plate format such as 96 or 384 well microplates. To monitor wnt signaling in intact cells human embryonic kidney hek293 cells were transiently transfected with a tcf lef luciferase reporter plasmid figure 1a as basal wnt signaling is relatively low in these cells figure s1a the gsk3 inhibitor chir99021 was used to enhance β catenin stabilization and activate wnt signaling the tankyrase inhibitor xav939 a known wnt signaling. The leading light wnt cell line assay reagents are intended for use with the leading light wnt reporter cell line which is the basis for a cell based luciferase activity test enz 61001 leading light wnt reporter assay starter kit suitable for a 96 well plate format.

The wnt signaling pathway helps animal cells to communicate with each other to coordinate the formation of tissues and organs. To enhance the reporter responsiveness these regulatory dna elements are synthesized in tandem repeats. S4b c producing a range of reporter activity similar. The pathway relies on a protein called wnt that is released from cells and binds to a receptor protein called frizzled on the surface of other cells to trigger changes in gene activation.

Wnt proteins bind to receptors on the cell surface initiating a signaling cascade that leads to stabilization and nuclear translocation of beta catenin. We found that both colon cancer cell lines induced canonical wnt signalling in a cell number dependent manner fig. 3a and supplementary fig. The system contains an engineered 3t3 mouse fibroblast cell line which expresses the firefly luciferase reporter gene.

Beta catenin then binds to tcf lef transcription factors in the nucleus leading to transcription and. There are various reporter transgenes that respond to wnt signals in intact animals and therefore as far as one can know reflect endogenous wnt signaling reviewed in barolo 2006 these reporters are based on a multimerized tcf binding site driving expression of lacz or gfp. Since the tcf lef h2b gfp transgenic is the first wnt reporter line to reveal the dynamics of wnt signaling activity in cells of the visceral endoderm of e5 5 embryos we focused on this stage to study the behavior of cells expressing the reporter. The active wnt reporter cell line is designed to monitor the activity of beta catenin based wnt signal transduction pathway.